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human genomic dna extraction kit  (Promega)
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Promega human genomic dna extraction kit
Human Genomic Dna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genomic Dnas Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genome dna extraction kit  (Promega)
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Promega genome dna extraction kit
Genome Dna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Wizard Dna Isolation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lysis solution wizard genomic dna purification kit  (Promega)
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Cell Lysis Solution Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega genomic dna purification kit wizard promega® a1120
Genomic Dna Purification Kit Wizard Promega® A1120, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genomic dna human blood  (Promega)
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Promega genomic dna human blood
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genomic Dna Human Blood, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard genomic dna purification kit # 1120  (Promega)
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Promega wizard genomic dna purification kit # 1120
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Genomic Dna Purification Kit # 1120, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
wizard genomic dna purification kit # 1120 - by Bioz Stars, 2026-02
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wizard genomic dna purification kit 12  (Promega)
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Promega wizard genomic dna purification kit 12
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Genomic Dna Purification Kit 12, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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silica column-based dna extraction kit reliaprep blood genomic dna miniprep system  (Promega)
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Promega silica column-based dna extraction kit reliaprep blood genomic dna miniprep system
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Silica Column Based Dna Extraction Kit Reliaprep Blood Genomic Dna Miniprep System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna rehydration solution wizard genomic dna purification kit  (Promega)
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Promega dna rehydration solution wizard genomic dna purification kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Dna Rehydration Solution Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Journal: BMC Genomics

Article Title: Human glycolipid transfer protein ( GLTP ) genes: organization, transcriptional status and evolution

doi: 10.1186/1471-2164-9-72

Figure Lengend Snippet: Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Article Snippet: Genomic DNA from human blood (Promega) or isolated from various cell lines (Sigma-Aldrich, GenElute™ mammalian genomic DNA miniprep kit) was bisulfite converted using manufacturer's recommendations (CpGenome Fast; Chemicon, Temecula, CA).

Techniques: Methylation, Clone Assay, Reverse Transcription Polymerase Chain Reaction, Combined Bisulfite Restriction Analysis Assay, Agarose Gel Electrophoresis, Amplification, Sequencing, Negative Control, Molecular Weight